Syncytia formation after surface expression of CHIKV-E1 in insect cells. Sf9-ET cells in cholesterol supplemented Sf900 II medium (pH = 6.4) were infected with Ac-6KE1, Ac-E3E2 and Ac-GFP. Cells were incubated 72 h post infection with medium of pH = 5.8, pH = 5.5 and pH = 5.0 for 2 min. Pictures were taken 4 h post induction. Syncytia are indicated with arrows.
Metz et al. Virology Journal 2011 8:353 doi:10.1186/1743-422X-8-353