Open Access Short report

Human-Phosphate-Binding-Protein inhibits HIV-1 gene transcription and replication

Thomas Cherrier126, Mikael Elias7, Alicia Jeudy1, Guillaume Gotthard2, Valentin Le Douce1, Houda Hallay1, Patrick Masson3, Andrea Janossy1, Ermanno Candolfi1, Olivier Rohr145, Eric Chabrière2* and Christian Schwartz14*

Author Affiliations

1 Institut de Parasitologie et Pathologie Tropicale, EA 4438, Université de Strasbourg, 3 rue Koeberlé, 67000 Strasbourg, France

2 Laboratoire URMITE - UMR 6236 Faculté de Médecine, 27, Bvd Jean Moulin, 13385 Marseille Cedex 5 France

3 Unité d'enzymologie, Département de Toxicologie, centre de recherche du service de santé des armées, 38702 la Tronche, France

4 IUT Louis Pasteur de Schiltigheim, 1 Allée d'Athènes, 67300 Schiltigheim, France

5 Institut Universitaire de France, 103 Bvd St-Michel, 75005 Paris, France

6 Cellular and Molecular Biology Unit, FUSAGx, Gembloux, Belgium

7 Weizmann Institute of Science, Biological Chemistry, Rehovot, Israel

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Virology Journal 2011, 8:352  doi:10.1186/1743-422X-8-352

Published: 15 July 2011

Abstract

The Human Phosphate-Binding protein (HPBP) is a serendipitously discovered lipoprotein that binds phosphate with high affinity. HPBP belongs to the DING protein family, involved in various biological processes like cell cycle regulation. We report that HPBP inhibits HIV-1 gene transcription and replication in T cell line, primary peripherical blood lymphocytes and primary macrophages. We show that HPBP is efficient in naïve and HIV-1 AZT-resistant strains. Our results revealed HPBP as a new and potent anti HIV molecule that inhibits transcription of the virus, which has not yet been targeted by HAART and therefore opens new strategies in the treatment of HIV infection.

Keywords:
HIV-1; HPBP; transcription; HAART