Open Access Case Report

Capacity building permitting comprehensive monitoring of a severe case of Lassa hemorrhagic fever in Sierra Leone with a positive outcome: Case Report

Jessica N Grove1, Luis M Branco12, Matt L Boisen3, Ivana J Muncy3, Lee A Henderson4, John S Schieffellin5, James E Robinson5, James J Bangura67, Mbalu Fonnie8, Randal J Schoepp9, Lisa E Hensley10, Alhassan Seisay6, Joseph N Fair7 and Robert F Garry1*

Author Affiliations

1 Tulane University Department of Microbiology and Immunology, New Orleans, LA, USA

2 Autoimmune Technologies, LLC, New Orleans, LA, USA

3 Corgenix Medical Corporation, Broomfield, CO, USA

4 Vybion, Inc., Ithaca, NY, USA

5 Tulane University Department of Pediatrics, Section of Infectious Disease, New Orleans, LA, USA

6 Ministry of Health and Sanitation Workplace Health, Sierra Leone

7 The Global Viral Forecasting Initiative, San Francisco, CA

8 Kenema Government Hospital Lassa Fever Ward, Kenema, Sierra Leone

9 Applied Diagnostics Branch, Diagnostic Systems Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA

10 Viral Therapeutics Branch, Virology Division, U.S. Army Medical Research Institute of Infectious Diseases Diagnostic Systems Division, Fort Detrick, MD, USA

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Virology Journal 2011, 8:314  doi:10.1186/1743-422X-8-314

Published: 20 June 2011

Additional files

Additional File 1:

Figure S1 - A comprehensive metabolic panel was obtained daily by Piccolo analysis. Fourteen metabolic indicators were measured in the serum of G-1180 daily after admission, through day 14 (with the exception of day 12), using a Piccolo comprehensive metabolic panel disk array (see Figure 3A for ALP, ALT, AST, BUN, TBil, Cre). G-1180 presented with low serum Na+, Cl-, and Ca2+ions, with K+, acidotic (low TCO2) and low Alb and TP levels. Contrastingly, G-1177 presented with high Na+, Cl-, K+, low Ca2+, and normal TCO2. Metabolic indicators were assayed in normal Sierra Leonean and U.S. normals, along with two samples each from G-1180 contacts -A and -B.

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Additional File 2:

Table S1 - Determination of LASV Ag specific immunoglobulin M and G endpoint titers with NP, GP1, GP2 and Z combination ELISA. IgM and IgG endpoint titers were determined for G-1180 and his contacts (A-L). G-1180 was admitted to KGH LFW with relatively low IgM and IgG titers that increased during hospitalization and upon convalescence. Contacts G-1180-A and -B presented to KGH LFW with significant IgM and IgG titers, suggesting previous exposure to LASV, and remained asymptomatic throughout the hospitalization timeline. Additionally, G-1180-C through -L displayed IgM, IgG, or dual immunoglobulin titers.

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