The plants (Tomato and Eggplant) were agro-infiltrated with various constructs as shown on the top of the figures, and the level of viral DNA accumulation was checked by semi-qPCR at 90 dpi using DNA-A specific abutting primers ToLCNDV-IN[IN:Nag:Egg: 09]. The amplification products (~2.7 kb) were separated on 1% agarose gel and are presented in panels (a) and (b) for tomato and eggplant respectively. The band intensities were quantified and the normalized values with respect to corresponding loading controls (ACTIN) are plotted as bar graphs in panels (c) and (d) respectively. The standard deviations shown are based on three independent experiments.
Pratap et al. Virology Journal 2011 8:305 doi:10.1186/1743-422X-8-305