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Open Access Highly Accessed Short report

An XMRV Derived Retroviral Vector as a Tool for Gene Transfer

Daniel Cervantes-Garcia12, Augusto Rojas-Martinez2 and David Camerini1*

Author Affiliations

1 Division of Infectious Diseases, Center for Virus Research and Institute for Immunology, University of California, Irvine, California, 92697-4068, USA

2 Department of Biochemistry and Molecular Medicine in the School of Medicine, Centro de Investigacion y Desarrollo en Ciencias de la Salud, Unidad de Terapia Genica y Celular, Universidad Autonoma de Nuevo Leon, Monterrey, Nuevo Leon, Mexico

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Virology Journal 2011, 8:284  doi:10.1186/1743-422X-8-284

Published: 8 June 2011

Abstract

Background

Retroviral vectors are widely used tools for gene delivery and gene therapy. They are useful for gene expression studies and genetic manipulation in vitro and in vivo. Many retroviral vectors are derived from the mouse gammaretrovirus, murine leukemia virus (MLV). These vectors have been widely used in gene therapy clinical trials. XMRV, initially found in prostate cancer tissue, was the first human gammaretrovirus described.

Findings

We developed a new retroviral vector based on XMRV called pXC. It was developed for gene transfer to human cells and is produced by transient cotransfection of LNCaP cells with pXC and XMRV-packaging plasmids.

Conclusions

We demonstrated that pXC mediates expression of inserted transgenes in cell lines. This new vector will be a useful tool for gene transfer in human and non-human cell lines, including gene therapy studies.

Keywords:
XMRV; retroviral vector; transduction