Figure 7.

Transcriptional analysis of DEV UL55 gene in infected cells in vitro. The transcriptional analysis of DEV UL55 in infected DEFs were performed by qRT-PCR. The transcriptional expression of DEV UL55 gene was normalized to a reference gene(β-actin) and relative to NTC control. The average relative content of the DEV UL55 gene transcripts were calculated at 0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 6.0, 8.0, 12, 16, 24, 36, 48, and 60 h post-infection using iCycler IQ 5.