Figure 7.

Dengue MAC-ELISA assay. In this modified IgM antibody capture ELISA (MAC-ELISA) assay a pool of sera from dengue virus-infected patients [containing antibodies against the 3 DENV sorotypes 1 (D1), 2 (D2) and 3 (D3)] were used with the following antigens: mock infected Tn5B cells, Tn5B cells infected with AcMNPV and Tn5B cells infected with vSynYFE. Positive control antigen is a pool of dengue virus (D1D2D3) and CN is the same assay with serum from a healthy individual. No reactivity was detected with vSynYFE-infected insect cells extracts treated with dengue-infected patients sera.