Table 1

Antigenicities of the MEAP with antibodies to B, C, D, E, G and I glycoprotein of HSV-2 and antigenicities of some B cell epitopes with antibodies to the MEAP

No.

Antibodies

EIA OD (450 nm) Value


gD26-18

Ab to MEAP

1.125 ± 0.041

gB2466-473

Ab to MEAP

0.977 ± 0.039

gC2216-223

Ab to MEAP

0.899 ± 0.031

gE2483-491

Ab to MEAP

1.102 ± 0.040

gG2572-579

Ab to MEAP

0.182 ± 0.011

gI2286-295

Ab to MEAP

0.792 ± 0.029

MEAP

Ab to gD2

1.099 ± 0.037

MEAP

Ab to gB2

0.986 ± 0.031

MEAP

Ab to gC2

0.893 ± 0.029

MEAP

Ab to gE2

1.013 ± 0.041

MEAP

Ab to gG2

0.201 ± 0.011

MEAP

Ab to gI2

0.876 ± 0.031

Control 1

Ab to MEAP

0.116 ± 0.011

Control 2

Ab to gD2

0.121 ± 0.010


The selected synthesized B cell epitopes were coated as antigens. The antibodies to the MEAP reacted with the epitopes. The antigenicities were detected by EIA. The MEAP was coated as an antigen. The antibodies to the B, C, D, E, G and I glycoproteins (ABcam) reacted with the MEAP, and the results can be seen in the Table. No. represents the number of the peptides and the MEAP. Control 1 is a peptide other from the HSV-2 glycoproteins. Control 2 is a antibody other than antibodies to the B, C, D, E, G and I glycoproteins of HSV-2. The O.D. mean values (at 450 nm) are shown in the Table.

Wang et al. Virology Journal 2011 8:232   doi:10.1186/1743-422X-8-232

Open Data