Table 1

Intra-assay performance of TaqMan qPCR with five different Chinese strains of CSFV

Samples

CT value

Result of intra-assay


1

2

3

4

5

6

7

8

9

Mean

SDs

CVs (%)


BJ1/08

21.23

21.39

21.42

21.43

21.53

21.60

23.10

23.14

23.17

21.67

0.366

1.62

BJ2/08

18.01

18.09

18.16

18.17

18.19

18.55

18.56

18.79

18.89

18.38

0.324

1.76

HeNXH2/98

21.11

21.14

21.17

21.40

21.44

23.02

23.17

23.28

23.55

21.70

0.557

2.45

FJFQ1/98

15.99

16.06

16.15

16.19

16.21

16.35

16.16

17.24

17.25

17.47

0.489

2.80

HeNBY1/96

24.70

25.04

25.06

25.41

25.05

25.81

25.82

26.29

26.39

26.42

0.599

2.27


9 repeats assay performed at the same time and under the same conditions

SD = standard deviation

CV = coefficient of variation

Liu et al. Virology Journal 2011 8:201   doi:10.1186/1743-422X-8-201

Open Data