DHV-1 IRES activity is modulated by downstream coding sequences. (A) Four additional reporter plasmids were constructed containing DHV-1 5'-UTR and 10, 40 or 60 nucleotides, corresponding to the coding region adjacent to the viral 5'-UTR (DHV-1 UTR+10nt, UTR+40nt, UTR+60nt, respectively). Dicistronic plasmids were in vitro transcribed and capped RNAs were individually added to Flexi rabbit reticulocyte lysate (RRL) system (Promega) and the in vitro translation of the reporter proteins was evaluated measuring 35S-methionine incorporation. (B) Dicistronic plasmids were transfected into vFT7-infected BHK-21 cells and the accumulation of fLUC protein expressed from the different constructs was determined.
Liu et al. Virology Journal 2011 8:147 doi:10.1186/1743-422X-8-147