DHV-1 IRES initiation does not require functional eIF4A. (A) Increasing amounts of different dominant negative mutants were added to RRL prior to the individual addition of the dicistronic capped RNAs indicated in the figure and translation of the reporter proteins was evaluated. (B) Purification and expression efficiency of the different eIF4A mutants used in (A). (C) The in vitro translation efficiency of the different dicistronic constructs indicated in the figure was evaluated in the RRL in the presence of increasing amounts of hippuristanol.
Liu et al. Virology Journal 2011 8:147 doi:10.1186/1743-422X-8-147