The DHV-1 IRES does not require intact eIF4G. (A) Flexi rabbit reticulocyte lysates were preincubated with or without recombinant FMDV L protease at a final concentration of 0, 1, 5, 25 μg/ml prior to the individual addition of the dicistronic capped RNAs indicated in the figure and translation of the reporter proteins was evaluated. (B) Western-blot showing the effect of recombinant FMDV L protease addition on eIF4GI.
Liu et al. Virology Journal 2011 8:147 doi:10.1186/1743-422X-8-147