Translation initiation of putative DHV-1 IRES is not inhibited by 4E-BP1. The dicistronic capped RNAs indicated in the figure were added to Flexi RRL in the presence of increasing amounts of purified recombinant 4E-BP1 protein (0, 9, 18 and 36 μg/ml) and the in vitro translation of the reporter proteins was evaluated measuring 35S-methionine incorporation. (B) Western-blot analysis of the recombinant 4E-BP1 protein added in the different conditions.
Liu et al. Virology Journal 2011 8:147 doi:10.1186/1743-422X-8-147