RT-PCR of total RNA from MCC samples. RT-PCR (a) was performed with gene-specific primers for LT1; nested PCR (b) was carried out with gene-specific primers (M1/2) within the LT1 amplicon. The numbers indicate the patients as in Table 1. M8, molecular weight marker (Roche, Milan, Italy); CTR (-), sterile water as negative control.
Paolini et al. Virology Journal 2011 8:103 doi:10.1186/1743-422X-8-103