Diametrically opposed effects of hypoxia and oxidative stress on two viral transactivators
1 Department of Microbiology, Immunology and Parasitology, LSU Health Sciences Center, 1901 Perdido Street, New Orleans, LA 70112, USA
2 Stanley S. Scott Cancer Center, 533 Bolivar Street, LSU Health Sciences Center, New Orleans, LA 70112, USA
Virology Journal 2010, 7:93 doi:10.1186/1743-422X-7-93Published: 10 May 2010
Additional file 1:
Zinc depletion, hypoxia, and oxidative stress do not affect basal transcription from the minimal TK promoter. (A) C33A cells transfected with TKp-luciferase (AGP47) were split 6 hours post-transfection such that one aliquot was exposed to 1 μM of TPEN for 18 hours prior to being assayed, (B) Cells transfected as in A were split 6 hours post-transfection and exposed to an additional 18 hours to normoxia (N) or hypoxia (H), (C) Cells transfected and split as in A were exposed to the indicated concentrations of paraquot, and (D) β-mercaptoethanol. Transactivation is expressed as a percent of expression under control conditions. Chelation of zinc, oxygen tension and oxidative stress did not significantly alter expression from the minimal HSV-1 promoter.
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