Table 1

HPV58 genome copy number in cotinuous 5 passages:

Passage

viral DNA copies/μg yeast DNA*


P6

5.13×107 ± 4.85×106

P7

4.09×107 ± 3.38×106

P8

6.35×107 ± 4.45×106

P9

5.93×107 ± 2.14×106

P10

7.09×107 ± 4.67×106


DNA was isolated from yeast culture from passage 5 to passage 10. Viral DNA copies were quantitated by quantative PCR. The standard curve was generated by serially diluted pEGFPN1-HPV58-Ura. There is 3-5 copies of viral DNA in per yeast cells.

*Values are mean ± standard deviation.

Li et al. Virology Journal 2010 7:368   doi:10.1186/1743-422X-7-368

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