Reactivity of gB-specific antibodies with HSV-1 ANG path virions treated with low pH. Extracellular HSV-1 ANG path or KOS virions were treated for 5 min at 37°C with medium buffered to the indicated pHs and were blotted immediately to nitrocellulose membrane. Blots were probed at pH 7.4 with the gB-specific MAb (A) H126, (B) SS10, (C) SS106 or (D) H1817 followed by horseradish peroxidase conjugated goat secondary antibody. The exposures shown highlight the pH thresholds.
Siekavizza-Robles et al. Virology Journal 2010 7:352 doi:10.1186/1743-422X-7-352