Additional file 3.
DNA fragmentation and MTT cytotoxicity analysis of HEK-293T/17 cells transfected with LASV gene constructs. A. Fragmentation assays were performed by resolving 2 μg of genomic DNA from transfected and untransfected cells on agarose gels. A low molecular weight DNA laddering effect consistent with apoptotic DNA fragmentation was not observed in any of the samples (n = 3). B. MTT cytotoxicity analysis of transfected cells, in 96-well format (n = 3). Vector only (pcDNA3.1+:intA), NP, GPC, and GPC-FLAG transfected cells did not display significant cytotoxicity when compared to untransfected controls (293T/17 cell ctrl) [p > 0.05]. Conversely, inclusion of the Z matrix gene, in native (Z) or 3'HIS-tagged format (Z-3'HIS), alone or in combination with any version of LASV GPC and/or NP resulted in significant reduction in MTT incorporation levels [p < 0.05 to p < 0.001, n = 3]. The numbered gel lanes in A. correspond to the bars in B. The p value for each transfection condition compared to the 293T/17 cell control is shown above the corresponding lane.
Format: PPTX Size: 1.4MB Download file
Branco et al. Virology Journal 2010 7:279 doi:10.1186/1743-422X-7-279