Additional file 2.
Transfection experiments with combinations of tagged and untagged Z, NP, and GPC constructs. HEK-293T/17 cells were transfected in 6-well plates as outlined in Methods, with combinations of LASV gene constructs. VLP were purified through 20% sucrose cushions and subjected to western blot analysis. Blots were probed with αGP1, αGP2, αFLAG M2, αHIS mAbs, or αNP PAb. Lane designations: 1. Z; 2. Z-3'HIS; 3. Z+GPC+NP; 4. Z+GPC-FLAG+NP; 5. Z-3'HIS+GPC+NP; 6. Z-3'HIS+GPC-FLAG+NP; 7. Z+GPC; 8. Z-3'HIS+GPC; 9. Z+GPC-FLAG; 10. Z-3'HIS+GPC-FLAG; 11. Z+NP; 12. Z-3'HIS+NP. The Z-3'HIS+GPC+NP combination consistently generated the highest VLP yields with corresponding incorporation of all three LASV genes.
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Branco et al. Virology Journal 2010 7:279 doi:10.1186/1743-422X-7-279