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Open Access Research

Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1

Yaming Chai, Hongyan Xiong*, Xiangyu Ma, Liqing Cheng, Guorong Huang and Zhonglin Rao Lin Zhang

Author Affiliations

Department of Epidemiology, Faculty of Preventive Medicine, Third Military Medical University, Chongqing 400038, China

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Virology Journal 2010, 7:255  doi:10.1186/1743-422X-7-255

Published: 28 September 2010

Abstract

Background

Bacteriophages (phages) are widespread in the environment and play a crucial role in the evolution of their bacterial hosts and the emergence of new pathogens.

Results

LSB-1, a reference coliphage strain, was classified as a member of the Podoviridae family with a cystic form (50 ± 5 nm diameter) and short tail (60 ± 5 nm long). The double stranded DNA was about 30 kilobase pairs in length. We identified its host range and determined the gp17 sequences and protein structure using shotgun analysis and bioinformatics technology.

Conclusions

Coliphage LSB-1 possesses a tailspike protein with endosialidase activity which is probably responsible for its specific enteroinvasive E.coli host range within the laboratory.