Table 2

Intra- and inter-assay co-efficient of variation for EBNA-1 and BHRF-1 QPCRs.

DNA Target

(copies/5 ul)

Mean CT

Mean R-G 6000™ Results

(copies/5ul)

Standard Deviation of R-G 6000™ Results

(copies/5ul)

Mean % Variation

COV

(%)

Mean R2


EBNA-1 Intra-Assay Variation (same day)


100,000

18.03

87,329

6,670

12.68%

7.64

0.991

10,000

21.28

11,735

3,092

26.30%

26.34

1,000

25.21

1,057

100

7.00%

9.50

100

29.10

103

38

32.12%

37.25

10

32.91

11

6

46.16%

57.50


EBNA-1 Intra-Assay Variation (different days)


100,000

16.94

89,643

8,164

11.00%

9.11

0.998

10,000

20.31

10,678

1,207

10.00%

11.31

1,000

23.85

1,133

129

16.00%

11.41

100

27.65

102

4

3.00%

3.90

10

31.42

10

2

17.88%

18.95


BHRF-1 Intra-Assay Variation (same day)


100,000

17.23

97,884

9,144

8.08%

9.34

0.994

10,000

20.91

9,852

542

4.45%

5.50

1,000

24.38

1,146

202

16.12%

17.64

100

28.43

94

19

17.70%

20.51

10

31.95

11

5

35.38%

41.91


BHRF-1 Intra-Assay Variation (different days)


100,000

18.05

105,387

4,621

6.02%

4.38

0.997

10,000

21.75

9,779

818

6.23%

8.37

1,000

25.23

1,042

141

11.63%

13.55

100

29.06

89

18

13.76%

19.88

10

32.66

12

2

25.30

16.53


EBNA-1 Inter-Assay Variation


100,000

19.87

101,644

14,058

10.99%

28.35

0.990

10,000

23.75

10,660

1,471

13.65%

13.80

1,000

27.68

1,084

191

18.67%

17.60

100

31.71

111

49

33.58%

43.75

10

35.86

12

9

65.03%

75.85


BHRF-1 Inter-Assay Variation


100,000

17.30

109,065

14,266

10.01%

13.08

0.990

10,000

21.49

9,209

2,154

16.84%

23.39

1,000

25.49

860

251

22.19%

29.15

100

29.01

108

49

35.53%

45.33

10

32.29

15

8

69.41%

57.23


Abbreviations: CT, cycle threshold; Mean % variation, average percentage variation between the calculated (Rotor-Gene results) and the given concentration (DNA target); COV, co-efficient of variation is the ratio of standard deviation to the mean; R2-value, square root of the correlation co-efficient - in quantitation PCR describes the percentage of the data which matches the hypothesis that the standards conform to a line of best fit.

Lay et al. Virology Journal 2010 7:252   doi:10.1186/1743-422X-7-252

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