Table 1 |
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Oligonucleotides used for EBV QPCR, beta-globin detection, construction of plasmid and PCR thermal cycling conditions. |
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|
Target |
Primer Name |
Oligonucleotide Sequence 5'-3' |
Amplicon Length |
GenBank Accession (position) |
Reference |
Optimised PCR Thermal Cycling Conditions |
|
|
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|
EBNA-1 |
QP1 |
GCC GGT GTG TTC GTA TAT GG |
213 bp |
(97174-97386) |
Stevens et al, 1999 |
95°C initial denaturation for 10 mins; 58°C annealing |
|
QP2 |
CAA AAC CTC AGC AAA TAT ATG AG |
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|
BHRF-1 |
EA-1F |
GGA GAT ACT GTT AGC CCT G |
208 bp |
(42105-42312) |
Custom |
98°C initial denaturation for 13 mins; 60°C annealing |
|
EA-2R |
GTG TGT TAT AAA TCT GTT CCA AG |
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|
Plasmid construct (randomised primers in bold) |
EA-F |
CTA TAT GTC TGC TTA CTC CGG CG /G GAG ATA CTG TTA GCC CTG |
554 bp |
N/A |
Custom |
95°C initial denaturation for 10 mins; 55°C annealing |
|
EB-R |
CGC CGG AGT AAG CAG ACA TAT AG /CAA AAC CTC AGC AAA TAT ATG AG |
95°C initial denaturation for 10 mins; 55°C annealing |
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|
Beta-Globin TAL57 |
BG-1F |
TAG CAA CCT CAA ACA GAC ACC A |
247 bp |
(171-417) |
Custom |
95°C initial denaturation for 10 mins; 61°C annealing |
|
BG-1R |
CAG CCT AAG GGT GGG AAA AT |
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|
|
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Abbreviations: EBNA-1, Epstein-Barr virus nuclear antigen-1; BHRF-1, BamHI fragment H rightward open reading frame-1; mins, minutes |
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|
Lay et al. Virology Journal 2010 7:252 doi:10.1186/1743-422X-7-252 |
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