Analytical sensitivity of NanoSign® Influenza A/B for novel influenza viruses. (A) The novel influenza A/California/12/2009(H1N1) viruses were cultured in the fertilized chicken embryo, harvested from the allantoic fluid, and purified to homogeneity using density gradient ultracentrifugation (3% sucrose), as described . The purified viruses were serially diluted in 2-fold manner and tested with the RDT kit. Bradford protein assay was used for the protein quantification. (B) The clinically isolated wild type novel influenza viruses were cultured in MDCK cell line. When the cytopathic effect was observed, the cultured viruses were harvested. The viral stocks were serially diluted in 2-fold manner and tested with the RDT kit. Hemagglutination assay was used for the titration of the viruses. w+, weak positive; CO, cut-off value.
Lee et al. Virology Journal 2010 7:244 doi:10.1186/1743-422X-7-244