Purification of the rabbit anti-tgK polyclonal antibody and Western blot assay. M represented standard protein molecular weight markers; M1 represented bicolor prestained protein markers. A. Purification of the rabbit anti-tgK polyclonal antibody. Lane1 represented that the polyclonal antibody was cursorily extracted by saturated ammonium sulfate; Lane 2 stood for the purified polyclonal antibody by ion exchange column chromatography. The heavy chain and light chain were approximately 55 KDa and 22 KDa, respectively. B. Western blot assay. Lane 1, Western blotting analysis showed that a specific band was recognized by rabbit anti-tgK monoclonal antibody, which was marked by the arrow; Lane 2, no band was detected by using rabbit preimmune serum.
Zhang et al. Virology Journal 2010 7:241 doi:10.1186/1743-422X-7-241