Figure 1.

Expression and purification of the tgK protein. M represented standard protein molecular weight markers. The arrow marked the purified tgK protein, which was approximately 34.0 KDa according to standard protein molecular weight markers. Lane 1 and Lane 2 respectively represented the uninduced and induced BL21 bacteria within pET-32b(+) plasmid; Lane 3 and Lane 4 respectively represented the uninduced and induced BL21 bacteria within pET-32b(+)/tgK plasmid; Lane 5 was the recombinant tgK protein purified by IMAC.

Zhang et al. Virology Journal 2010 7:241   doi:10.1186/1743-422X-7-241
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