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Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus

Shunchuan Zhang1, Jun Xiang1, Anchun Cheng123*, Mingshu Wang12*, Xin Li1, Lijuan Li1, Xiwen Chen2, Dekang Zhu12, Qihui Luo2 and Xiaoyue Chen123

Author Affiliations

1 Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, 46# Xinkang Road, Ya'an, Sichuan 625014, China

2 Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya'an 625014, China

3 Epizootic Diseases Institute of Sichuan Agricultural University, Ya'an, Sichuan 625014, China

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Virology Journal 2010, 7:241  doi:10.1186/1743-422X-7-241

Published: 17 September 2010

Abstract

Duck virus enteritis (DVE) is an acute, contagious herpesvirus infection of ducks, geese, and swans, which has produced significant economic losses in domestic and wild waterfowl. With the purpose of decreasing economic losses in the commercial duck industry, studying the unknown glycoprotein K (gK) of DEV may be a new method for preferably preventing and curing this disease. So this is the first time to product and purify the rabbit anti-tgK polyclonal antibody. Through the western blot and ELISA assay, the truncated glycoprotein K (tgK) has good antigenicity, also the antibody possesses high specificity and affinity. Meanwhile the rabbit anti-tgK polyclonal antibody has the potential to produce subunit vaccines and the functions of neutralizing DEV and anti-DEV infection because of its neutralization titer. Indirect immunofluorescent microscopy using the purified rabbit anti-tgK polyclonal antibody as diagnostic antibody was susceptive to detect a small quantity of antigen in tissues or cells. This approach also provides effective experimental technology for epidemiological investigation and retrospective diagnose of the preservative paraffin blocks.