Analysis of vascular permeability and HCT during YFV infection in hamsters. Groups of animals were infected with ~20 cell culture infectious doses (CCID50) of YFV or sham-infected. On specified days of the infection (days 3-7), groups of YFV-infected hamsters (n = 5) and groups of sham-infected animals (n = 3) were injected retro-orbitally with EBD following collection of whole blood for evaluation of HCT. Vascular leak into the (A) liver, (B) spleen, (C) kidney, and (D) small intestine tissues, and (E) HCT were evaluated. Vascular permeability data are expressed as the ratio of absorbance/g of tissue:absorbance of the diluted matching serum sample. SI, sham-infected. *P < 0.05, ***P < 0.001 compared to the pool of sham-infected animals using ANOVA with the Newman-Keuls multiple comparison test.
Gowen et al. Virology Journal 2010 7:240 doi:10.1186/1743-422X-7-240