Analysis of vascular permeability during PICV infection in hamsters. Groups of animals were infected with ~5 plaque-forming units of PICV, with the exception of 4 hamsters that were processed at the time of infection to establish the day 0 baseline reading. On each day of the infection (days 1-8), a group of animals was injected retro-orbitally with EBD-PBS and vascular leak into the (A) liver, (B) spleen, (C) kidney, and (D) lung tissues was evaluated. Data are expressed as the ratio of absorbance/g of tissue:absorbance of the diluted matching serum sample. For days 1-6, there were 5 hamsters/group. Days 7 and 8 had 6 animals/group, but due to the death of one animal in each group prior to time of sacrifice, the analysis is based on 5 hamsters for these groups. *P < 0.05, **P < 0.01 compared to day 0 animals using one-way analysis of variance (ANOVA) with the Newman-Keuls multiple comparison test.
Gowen et al. Virology Journal 2010 7:240 doi:10.1186/1743-422X-7-240