Effect of HDAC4 knockdown on the efficiency of integration and PIR. (A) HDAC4 was suppressed in HeLa cells using an siRNA treatment two days in a row (see "Experimental Procedures"). Two days after the first siRNA transfection, cells were infected with the HIV-1- based vector. DNA was extracted 3 days post-infection and analyzed by Alu-PCR (see "Experimental Procedures"). +Alu - DNA was analyzed using Alu-PCR, -Alu - a negative control, the Alu primer was left out in the first round of PCR. M - uninfected cells, Ci - cells transfected with control siRNA and infected with the vector, Hi - cells transfected with HDAC4 siRNA and infected with the vector. (B) HDAC4 levels in cells transfected with control (Ci) and HDAC4 siRNA (Hi).
Smith et al. Virology Journal 2010 7:237 doi:10.1186/1743-422X-7-237