Table 1

Sequence of HPV type-specific L1 primers and probes used for qPCR.

Oligonucleotide

Sequence (5'→3')

Concentration/Rx

HPV6L1F

TGGGGTAATCAACTGTTTGTTACTGTGGTA

400 nM

HPV6L1R

GCATGTACTCTTTATAATCAGAATTGGTGTATGTG

400 nM

HPV6L1probe

Cy5-GACATTATGTGCATCCGTAACTAC-BHQ2

200 nM

HPV11L1F

CTGGGGAAACCACTTGTTTGTTACTGTG

400 nM

HPV11L1R

CGCATGTATTCCTTATAATCTGAATTAGTGTATGTA

400 nM

HPV11L1probe

TexasRed-GACACTATGTGCATCTGTGTCTAA-BHQ1

800 nM

HPV16L1F

TTGTTGGGGTAACCAACTATTTGTTACTGTT

400 nM

HPV16L1R

CCTCCCCATGTCTGAGGTACTCCTTAAAG

400 nM

HPV16L1probe

6FAM-GTCATTATGTGCTGCCATATCTACTTC-TAMRA

400 nM

HP18L1F

GCATAATCAATTATTTGTTACTGTGGTAGATACCACT

400 nM

HP18L1R

GCTATACTGCTTAAATTTGGTAGCATCATATTGC

400 nM

HPV18L1probe

HEX-AACAATATGTGCTTCTACACAGTCTCCTGT-BHQ2

100 nM

HPVE1F1

ANANGCTGTGCAKGNNCTAAAACGAAG

300 nM

HPVE1R1

AGTTTCCACTTCAGTATTGCCATA

300 nM

HAPBF

TGAAGGTGGAGGACATTCCTCTA

400 nM

HAPBR

CTGGAATTGCGATTTCTGGTAA

400 nM

HAPBprobe

Cyan500-CGAGAATCACCCTGCCAGACTTCCGT-BBQ

100 nM

Seaman et al. Virology Journal 2010 7:194   doi:10.1186/1743-422X-7-194