Sequence and location of HPV type-specific L1 primers used for qPCR. The top line represents a schematic diagram of the HPV L1 gene. Black boxes indicate the coding regions for the 5 hypervariable loop regions. The arrowheads indicate the position of the MY09/11primer pair used in traditional nested PCR. The coding region for the hypervariable loop V region is expanded below the schematic. Shown is an alignment of the HPV types identified in this study (HPV6, 11, 16 and 18) as well as other major oncogenic HPV types (HPV 31, 33, 45, 52, and 58). The type-specific primer sequences used for qPCR are underlined. Sequences corresponding to type-specific probes are boxed. Arrows indicate the position and sequence of the GP5+/GP6+ primer pair used in traditional nested PCR.
Seaman et al. Virology Journal 2010 7:194 doi:10.1186/1743-422X-7-194