Validation of microarray results by quantitative real-time PCR. Copy number of eight common cytokines and a house keeping gene (B2M) were calculated based on RT-PCR Ct values. Fold change of genes expression is plotted on Y-axis after normalization to mock-treated samples. Results plotted to compare calculated fold change in expression of each gene at 3 hpi (A) or at 7 hpi (B) using Microarray (grey bars) and RT-PCR (black bars).
Alkhalil et al. Virology Journal 2010 7:173 doi:10.1186/1743-422X-7-173