Figure 5.

Prunella extracts inhibit EIAV infectivity primarily by acting on viral particles. A) Ability of cell-associated Prunella extracts to inhibit EIAV infection. Extracts were incubated with ED cells and removed prior to the addition of EIAV. EIAV infection was evaluated at 40 h following infection. B) Ability of Prunella extracts to inhibit EIAV. 2.2 x 105 EIAVWSU5 particles were incubated with 25, 50 or 100 μg/mL extract from Prunella Ames 27664 extract for 10 minutes at room temperature. Viral stocks were diluted in media containing the appropriate concentration of extract and plated onto ED cells. Forty h following infection the cells were fixed, immunostained for EIAV antigen and enumerated. Shown are the numbers of infectious virions/ml. Data represent the average and standard error of three experiments performed in duplicate (A) or triplicate (B). Extracts significantly reduced EIAV virion infectivity at all concentrations tested.

Brindley et al. Virology Journal 2009 6:8   doi:10.1186/1743-422X-6-8
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