EIAV inhibition by water extracts of Prunella Ames 27664 and 27748 is not cell type or strain specific. A) EIAV infections of UVECs. DMSO and water extracts of Ames 27664 and 27748 were diluted in media to 0.1% (33 μg/mL of Ames 27664 or 31.2 μg/mL of Ames 27748) or 0.2% (66 μg/mL of 27664 or 62.4 μg/mL of 27748). EIAVWSU5 virus was added to the diluted extracts and immediately used to infect eUVECs. B) Inhibition of infection of four EIAV strains was evaluated in ED cells in the presence of 0.2% P. vulgaris aqueous extracts of Ames 27664 and 27748 or DMSO. Forty h following infection, cells were immunostained for EIAV antigen. Parallel cultures that were treated with extract, but not infected were evaluated for cell viability. Shown are the ratios of the values in the presence of the extracts divided by the DMSO control. Shown are the averages and standard errors of three experiments performed in triplicate. *, p < 0.05; **, p < 0.001.
Brindley et al. Virology Journal 2009 6:8 doi:10.1186/1743-422X-6-8