Lactoferrin has inhibitory activity against HSV-1 infection also after viral adsorption. To study the lactoferrin effect on post-attachment events, wt HSV-1 and mutant viruses gC-39 and Rid1 were adsorbed to cells at 4°C, cultures were washed and human non-iron-saturated Lf (apoLf), iron-saturated Lf (satLf) or lysozyme (hLz) was added. The relative amount of viral plaques, in comparison to untreated cultures, is shown. Error bars represent standard deviations of three separate experiments. Statistical significance in the level of inhibition: * = p < 0.05, ** = p < 0.005, mutant viruses compared to wt KOS. Only results with the highest concentration of hLz used are shown.
Välimaa et al. Virology Journal 2009 6:53 doi:10.1186/1743-422X-6-53