Pretreatment of cell cultures with lactoferrin but not lysozyme inhibited HSV-1 infection. Vero cell cultures were pretreated with human non-iron-saturated Lf (apoLf), iron-saturated Lf (satLf) or lysozyme (hLz) for 24 h prior to inoculation. To exclude lactoferrin and lysozyme effect on HSV-1 virion, these supplements were removed from the media by washing before inoculation of cultures with wt HSV-1 and mutant viruses gC-39 and Rid1. The relative amount of viral plaques, in comparison to untreated cultures, is shown. Error bars represent standard deviations of three separate experiments. Statistical significance in the level of inhibition: * = p < 0.05, ** = p < 0.005, mutant viruses compared to wt KOS. Only results with the highest concentration of hLz used are shown.
Välimaa et al. Virology Journal 2009 6:53 doi:10.1186/1743-422X-6-53