Lactoferrin but not lysozyme neutralized HSV-1. Wt HSV-1 and mutant viruses gC-39 and Rid1 were incubated for 30 min at 37°C with different concentrations of human non-iron-saturated Lf (apoLf), iron-saturated Lf (satLf) or lysozyme (hLz) prior to inoculation of Vero cell cultures. Preincubated virus/hLf mixtures were diluted 1:10 upon addition to the cell culture so that the inoculum contained 1–50 μg/ml of hLf. Therefore the observed inhibition could not have resulted from the hLf effect on the cells. The relative amount of viral plaques, in comparison to untreated cultures, is shown. Error bars represent standard deviations of three separate experiments. Statistical significance in the level of inhibition: * = p < 0.05, ** = p < 0.005, mutant viruses compared to wt KOS. Only results with the highest concentration of hLz used are shown.
Välimaa et al. Virology Journal 2009 6:53 doi:10.1186/1743-422X-6-53