A correction for this article has been published in Virology Journal 2009, 6:80

Research

Presence of necrotic strains of Potato virus Y in Mexican potatoes

Victoriano Roberto Ramírez-Rodríguez¹ , Katia Aviña-Padilla¹ , Gustavo Frías-Treviño² , Laura Silva-Rosales¹ and Juan Pablo Martínez-Soriano¹

¹  Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Campus Guanajuato, km. 9.6 libramiento norte, carretera Irapuato-León, 36821 Irapuato, Guanajuato, Mexico

²  Universidad Autónoma Agraria Antonio Narro, Departamento de Parasitología, Buenavista, Saltillo, Coahuila, Mexico

author email corresponding author email

Virology Journal 2009, 6:48doi:10.1186/1743-422X-6-48

Published:	6 May 2009

Abstract

As part of a routine screening for the possible presence of the necrotic strains of potato virus Y affecting potatoes in Mexico, five PVY isolates were submitted to biological and molecular analysis. Considering their serological properties, two belong to the common strain (O) and three to the necrotic strain (N). All the isolates induced vein necrosis in Nicotiana tabacum. To characterize the isolates, 5' NTR and P1 genes were sequenced and compared with sequences from GenBank database. Nucleotide sequence similarity ranged from 47–100% in the 5' NTR and from 63–100% in the P1 coding region. The lowest amino acid similarity between sequences of P1 gene was 55%. In phylogenetic trees of 5'NTR analysis, two PVY^O Mexican isolates clustered with other PVY^O isolates. In turn, the three PVY^N isolates grouped with PVY^N-NTN isolates. The phylogenetic analysis of P1 sequences (nucleotide and amino acid) showed two PVY^O isolates grouping next to N-NTN cluster. A detailed analysis of the PVY^O isolates showed two potential recombination regions in the P1 gene, in contrast to 5'NTR where no recombination was detected.