 Short reportThe genome and proteome of a virulent Escherichia coli O157:H7 bacteriophage closely resembling Salmonella phage Felix O1Andre Villegas1 1 Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, 110 Stone Road West, Guelph, ON, N1G 3W4, Canada 2 Centre for Biologics Research, Biologics and Genetic Therapies Directorate, Health Canada, 251 Sir Frederick Banting Driveway, Tunney's Pasture, Ottawa, ON, K1A 0K9, Canada 3 Department of Molecular & Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada 4 Pro-Lab Developments Inc, 200 Gerrard Street E, Suite 300, Toronto, ON, M5A 2E6, Canada 5 Département de Biologie médicale, Faculté de médecine, Université Laval, Québec, QC, G1K 7P4, Canada 6 Public Health Agency of Canada, National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, 1015 Arlington Street, Winnipeg, MB, R3E 3R2, Canada
Virology Journal 2009, 6:41doi:10.1186/1743-422X-6-41
AbstractBased upon whole genome and proteome analysis, Escherichia coli O157:H7-specific bacteriophage (phage) wV8 belongs to the new myoviral genus, "the Felix O1-like viruses" along with Salmonella phage Felix O1 and Erwinia amylovora phage φEa21-4. The genome characteristics of phage wV8 (size 88.49 kb, mol%G+C 38.9, 138 ORFs, 23 tRNAs) are very similar to those of phage Felix O1 (86.16 kb, 39.0 mol%G+C, 131 ORFs and 22 tRNAs) and, indeed most of the proteins have their closest homologs within Felix O1. Approximately one-half of the Escherichia coli O157:H7 mutants resistant to phage wV8 still serotype as O157:H7 indicating that this phage may recognize, like coliphage T4, two different surface receptors: lipopolysaccharide and, perhaps, an outer membrane protein. |
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