Figure 5.

Comparison of rAAV-IGF-I and wt AAV2 viral yield. Wild type AAV2 was prepared by combining pAV1 (10 μg) and pHelper (10 μg) to transfect 293 cells. rAAV-IGF-I was prepared by combining the three plasmids: pAAV-RC/p5 (10 μg), pAAV-IGF-I (10 μg) and pHelper (10 μg) for transfection. rAAV-IGF-I titer and wt AAV2 titer are expressed as viral packaged genomes/10-cm plate. Data represent the means and SDs from three independent experiments.