Research

Evidence of recombination in Hepatitis C Virus populations infecting a hemophiliac patient

Pilar Moreno¹ , Macarena Alvarez¹ , Lilia López^1,2 , Gonzalo Moratorio¹ , Didier Casane³ , Matías Castells¹ , Silvia Castro⁴ , Juan Cristina¹ and Rodney Colina¹

¹  Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay

²  Servicio Nacional de Sangre, Montevideo, Uruguay

³  Laboratoire Evolution Génomes Spéciation, CNRS 91198 Gif-sur-Yvette, France

⁴  Cátedra de hemoterapia, Hospital de Clínicas, Montevideo, Uruguay

author email corresponding author email

Virology Journal 2009, 6:203doi:10.1186/1743-422X-6-203

Published:	18 November 2009

Abstract

Background/Aim

Hepatitis C virus (HCV) infection is an important cause of morbidity and mortality in patients affected by hereditary bleeding disorders. HCV, as others RNA virus, exploit all possible mechanisms of genetic variation to ensure their survival, such as recombination and mutation. In order to gain insight into the genetic variability of HCV virus strains circulating in hemophiliac patients, we have performed a phylogenetic analysis of HCV strains isolated from 10 patients with this kind of pathology.

Methods

Putative recombinant sequence was identified with the use of GARD program. Statistical support for the presence of a recombination event was done by the use of LARD program.

Results

A new intragenotypic recombinant strain (1b/1a) was detected in 1 out of the 10 hemophiliac patient studied. The recombination event was located at position 387 of the HCV genome (relative to strain AF009606, sub-type 1a) corresponding to the core gene region.

Conclusion

Although recombination may not appear to be common among natural populations of HCV it should be considered as a possible mechanism for generating genetic diversity in hemophiliacs patients.