Table 3

Vaginal application of FSL-1 in mice induced a specific cytokine profile.

6 h Post FSL-1 application (pg/lavage ± SEM)


Cytokinea

FSL-1

DPBS vehicle


IL-1α

1083.2 ± 143.5b

527.0 ± 65.7

IL-1β

1157.0 ± 320.2c

169.2 ± 46.3

IL-2

34.8 ± 5.2d

BDL(3.9)e

IL-6

418.5 ± 94.1b

35.4 ± 8.5

IL-12(p40)

104.2 ± 10.5d

44.9 ± 3.8

IL-12(p70)

64.0 ± 19.1c

17.1 ± 3.1

G-CSF

5157.0 ± 1095.0c

2208.0 ± 433.1

GM-CSF

198.6 ± 44.6b

22.6 ± 8.7

IFNα

30.3 ± 4.4d

0.7 ± 0.6

IFNβ

445.3 ± 93.2b

13.8 ± 3.5

IFNγ

76.8 ± 18.8c

BDL(43.1)

MIP-1α

2770.7 ± 484.0b

440.0 ± 127.0

MIP-1β

4140.0 ± 570.5c

2232.0 ± 218.5

TNF-α

1109.6 ± 212.7b

232.6 ± 71.6


a The indicated cytokines were found to be induced by FSL-1 application by BioPlex or ELISA as indicated in the methods. Data are presented as mean ± SEM of five replicates from two independent experiments.

b p < 0.01 compared to DPBS vehicle (Student's t-test).

c p < 0.05 compared to DPBS vehicle (Student's t-test).

d p < 0.001 compared to DPBS vehicle (Student's t-test).

e BDL; Below Detection Limit (for statistical comparisons with these cytokines the value for the lowest standard of each cytokine was utilized).

Rose et al. Virology Journal 2009 6:195   doi:10.1186/1743-422X-6-195

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