Research

Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo

Jin-Long Yang^1,2 , An-Chun Cheng^2,3 , Ming-Shu Wang^2,3 , Kang-Cheng Pan^2,3 , Min Li² , Yu-Fei Guo² , Chuan-Feng Li² , De-Kang Zhu^2,3 and Xiao-Yue Chen^2,3

¹  Chongqing Academy of Animal Science, Chongqing 402460, Chongqing, China

²  Avian Diseases Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan, China

³  Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Yaan 625014, Sichuan Province, China

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Virology Journal 2009, 6:142doi:10.1186/1743-422X-6-142

Published:	15 September 2009

Abstract

Background

Goose parvovirus (GPV) is a Dependovirus associated with latent infection and mortality in geese. Currently, it severely affects geese production worldwide. The objective of this study was to develop a fluorescent quantitative real-time polymerase chain reaction (PCR) (FQ-PCR) assay for fast and accurate quantification of GPV DNA in infected goslings, which can aid in the understanding of the regular distribution pattern and the nosogenesis of GPV in vivo.

Results

The detection limit of the assay was 2.8 × 10¹ standard DNA copies, with a sensitivity of 3 logs higher than that of the conventional gel-based PCR assay targeting the same gene. The real-time PCR was reproducible, as shown by satisfactory low intraassay and interassay coefficients of variation.

Conclusion

The high sensitivity, specificity, simplicity, and reproducibility of the GPV fluorogenic PCR assay, combined with a high throughput, make this method suitable for a broad spectrum of GPV etiology-related applications.