Table 2

Effects of inhibitors on kinetics parameters of A/PR/8/34 (H1N1) sialidase activity

Kinetic parameter

No inhibitor

OC

(4 nM)

PVP-I

(75 μg/ml)

DANA

(5 μM)


Vmax(μmol/l.min)

1.70

1.68

1.22

1.69

Km (μmol/l)

14.66

103.20

77.72

184.10

Km (μg/ml)a

7.17

-

-

-

Vmax/Km(1/min)

0.12

0.02

0.02

0.01


Type of inhibition

-

Competitive

Mixed

Competitive


Ki(nM)

-

0.66

-

432.60

Ki (μg/ml)

-

-

11.74

-

Kis (μg/ml)

-

-

190.63

-


Km/Ki

-

2.21 × 104

0.61

33.90

Km/Kis

-

-

0.04

-


aMW of MUNA is 489.39.

Sriwilaijaroen et al. Virology Journal 2009 6:124   doi:10.1186/1743-422X-6-124

Open Data