The complete genomic sequence of an in vivo low replicating BLV strain

doi:10.1186/1743-422X-6-120

Virology Journal

Volume 6

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Dube S
Abbott L
Dube DK
Dolcini G
Gutierrez S
Ceriani C
Juliarena M
Ferrer J
Perzova R
Poiesz BJ

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Dube S
Abbott L
Dube DK
Dolcini G
Gutierrez S
Ceriani C
Juliarena M
Ferrer J
Perzova R
Poiesz BJ
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The complete genomic sequence of an in vivo low replicating BLV strain

Syamalima Dube¹ , Lynn Abbott¹ , Dipak K Dube¹ , Guillermina Dolcini²^,3 , Silvina Gutierrez²^,3 , Carolina Ceriani²^,3 , Marcela Juliarena²^,4 , Jorge Ferrer⁵ , Raisa Perzova¹ and Bernard J Poiesz¹

¹Department of Medicine, Upstate Medical University, Syracuse, New York 13210, USA

²Universidad Nacional del Centro de la Provincia de Buenos Aires, Facultad de Ciencias Veterinarias, Tandil, Argentina

³Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina

⁴Comisión de Investigaciones Científicas y Técnicas de la Provincia de Buenos Aires (CIC), Argentina

⁵Comparative Leukemia and Retroviruses Unit, New Bolton Center, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA

author email corresponding author email

Virology Journal 2009, 6:120doi:10.1186/1743-422X-6-120


Published:	3 August 2009

Abstract

DNA was extracted from lamb lymphocytes that were infected in vivo with a BLV strain after inoculation with the peripheral blood mononuclear cells from a persistently sero-indeterminate, low viral load, BLV-infected Holstein cow (No. 41) from Argentina. The DNA was PCR amplified with a series of overlapping primers encompassing the entire BLV proviral DNA. The amplified BLV ARG 41 DNA was cloned, sequenced, and compared phylogenetically to other BLV sequences including an in vivo high replicating strain (BLV ARG 38) from the same herd in Argentina. Characterization of BLV ARG 41's deduced proteins and its relationship to other members of the PTLV/BLV genus of retroviruses are discussed.