Research
The complete genomic sequence of an in vivo low replicating BLV strain
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* Corresponding author: Bernard J Poiesz poieszb@upstate.edu
1 Department of Medicine, Upstate Medical University, Syracuse, New York 13210, USA
2 Universidad Nacional del Centro de la Provincia de Buenos Aires, Facultad de Ciencias Veterinarias, Tandil, Argentina
3 Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
4 Comisión de Investigaciones Científicas y Técnicas de la Provincia de Buenos Aires (CIC), Argentina
5 Comparative Leukemia and Retroviruses Unit, New Bolton Center, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA
Virology Journal 2009, 6:120 doi:10.1186/1743-422X-6-120
Published: 3 August 2009Abstract
DNA was extracted from lamb lymphocytes that were infected in vivo with a BLV strain after inoculation with the peripheral blood mononuclear cells from a persistently sero-indeterminate, low viral load, BLV-infected Holstein cow (No. 41) from Argentina. The DNA was PCR amplified with a series of overlapping primers encompassing the entire BLV proviral DNA. The amplified BLV ARG 41 DNA was cloned, sequenced, and compared phylogenetically to other BLV sequences including an in vivo high replicating strain (BLV ARG 38) from the same herd in Argentina. Characterization of BLV ARG 41's deduced proteins and its relationship to other members of the PTLV/BLV genus of retroviruses are discussed.