Figure 3.

Peptide-specific cytotoxic T cell responses against the HIV-1 Gag antigen in mice immunized with Gag or with the combination Gag and Tat. Splenocytes from each group were re-stimulated in vitro with the indicated peptides. After 5 days, T cell cultures were tested against P815 cells (ratio 50:1) treated with the indicated peptides. Results from mice immunized with Gag (empty bars) and from mice immunized with Gag and Tat (filled bars) are expressed as % specific lysis calculated by subtracting the lysis of untreated P815 cells (always below 3%). The mean +/- SD of the results from three independent experiments, performed in triplicate, is shown.