Figure 2.

Southern blotting analysis of pOka-BAC and pOka-BACΔ58. DNA. Purified pOka-BAC DNA and pOka-BACΔ58 DNA were digested by BamHI and loaded onto a 0.5% TBE agarose gel. The fragments recognized by the ORF58, ORF62/71 and Km^r probes (right) are indicated by arrowheads in the photograph (left). Southern blotting was performed using ORF58, ORF62/71, or the Km^r gene as a probe.