Figure 1.

Crystallogenesis of histidine-tagged isoform 3 of EED. (A), Platelet-like crystals of EED3-H6 protein of 441 residues, obtained in suspended drop in 0.1 M MES buffer pH 6.0, 40 % MPD. (B), Solubilization of the crystals and analysis by SDS-PAGE and Coomassie blue staining. Lane 1 : solution of purified EED3-H6 (10 mg/mL) used for crystallogenesis (protein load : 50 μg). Lane 2 : protein content of solubilized single crystal. MW : markers of protein molecular mass, indicated in kilodaltons (kDa) on the right side of the panel.

Rakotobe et al. Virology Journal 2008 5:32   doi:10.1186/1743-422X-5-32
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