Resolution:
standard / ## Figure 6.
Growth of transduced HaCaT cells in soft agar. Cells were plated on soft agar and overlayed with DMEM containing 50 ng/mL EGF. After
21 days growth dishes were stained with Cristal violet and the colony number (A) or
colony size (B, C) was calculated. A) average values for the number of colonies generated
in each different transfectant. Error bar encompass 95% confidence interval of themean.
Inset A) P-values for a one-tail Student's t-test. Values with statistically significant
differences have been shadowed. B) values for the Huber central estimator for the
colony size -arbitrary units- in each different transfectant. Error bar encompass
the median absolute deviation. The vertical scale has been broken in the interval
0–4 since the smallest colony size detectable with the hardware/software employed
was four units. Dashed line marks the 95% confidence value for the colony size in
the HaCaT control cell lines. Inset B) P-values for a Wilcoxon-Mann-Whitney test;
H0 = median values of both populations are similar. Values with statistically significant
differences have been shadowed. C) Example showing the histograms for distribution
of colony size in control HaCaT cells -black- and in hE5 transfectants -red. Continuous
lines mark the corresponding values for the average of the population. Dashed lines
mark the corresponding values for the Huber central estimators.
Lewis |