Research

The genome and proteome of the Kluyvera bacteriophage Kvp1 – another member of the T7-like Autographivirinae

Erika J Lingohr¹ , Andre Villegas¹ , Yi-Min She² , Pieter-Jan Ceyssens³ and Andrew M Kropinski^1,4

¹Public Health Agency of Canada, Laboratory for foodborne Zoonoses, Guelph, ON N1G 3W4, Canada

²Department of Chemistry, Queen's University, Kingston, Ontario K7L 3N6, Canada

³Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium

⁴Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada

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Virology Journal 2008, 5:122doi:10.1186/1743-422X-5-122

Published:	20 October 2008

Abstract

Background

Kluyvera, a genus within the family Enterobacteriaceae, is an infrequent cause of human infections. Bacteriophage Kvp1, the only bacteriophage isolated for one of its species, Kluyvera cryocrescens, is a member of the viral family Podoviridae.

Results

The genome of Kvp1, the first Kluyvera cryocrescens-specific bacteriophage, was sequenced using pyrosequencing (454 technology) at the McGill University and Genome Québec Innovation Centre. The two contigs were closed using PCR and the sequence of the terminal repeats completed by primer walking off the phage DNA. The phage structural proteome was investigated by SDS-PAGE and mass spectrometry.

Conclusion

At 39,472 bp, the annotated genome revealed a closer relationship to coliphage T3 than T7 with Kvp1 containing homologs to T3 early proteins S-adenosyl-L-methionine hydrolase (0.3) and protein kinase (0.7). The quantitative nature of the relationships between Kvp1 and the other members of the T7-like virus genus (T7, T3, φA1122, φYeO3-12, Berlin, K1F, VP4 and gh-1) was confirmed using CoreGenes.